Stains & CD markers
Phosphohistone H3
Copyright: 2002-2025, PathologyOutlines.com, Inc.
PubMed Search: Phosphohistone H3 [title]
Phosphohistone H3
Author: Nat Pernick, M.D.
Last author update: 1 June 2005
Last staff update: 23 July 2021
Copyright: 2002-2025, PathologyOutlines.com, Inc.
PubMed Search: Phosphohistone H3 [title]
Table of Contents
Definition / general | Interpretation | Uses by pathologists | Microscopic (histologic) images | Positive staining - diseaseCite this page: Pernick N. Phosphohistone H3. PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/stainsphosphohistoneh3.html. Accessed March 30th, 2025.
Definition / general
- Core histone protein that is major constituent of chromatin; marker of cells in late G2 and M phase
Interpretation
- Nuclear stain
- Count phosphohistone H3+ objects (nuclei and mitoses) in 10 adjacent fields with 40x objective, in invasive epithelial areas with highest phosphohistone H3 staining
- Ignore nuclei with fine granular staining
Uses by pathologists
- Identify mitotic activity in tumor cells; use for counting mitotic figures
- Strong prognostic value in lymph node negative breast cancer patients < 71 years old (Mod Pathol 2007;20:1307, Mod Pathol 2011;24:502)
- Strong prognostic value (<13 versus 13+) in basal-like and triple negative, lymph node negative breast cancer patients (Cell Oncol 2009;31:261)
- Prognostic value in meningiomas (Am J Clin Pathol 2007;128:118, Am J Surg Pathol 2004;28:1532)
Microscopic (histologic) images
Images hosted on other servers:
Normal colonic mucosa
Ki67, phosphohistone H3 and survivin
Grade 3 breast carcinoma
Thick cutaneous melanomas
Positive staining - disease
- Mitotic activity
