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Skin-nontumor / Clinical Dermatology



Reviewer: Cecilia Rosales, M.D, Baylor College (see Reviewers page)
Revised: 24 August 2011, last major update August 2010
Copyright: (c) 2002-2011, PathologyOutlines.com, Inc.


● Breadloafing (serial section) of ellipses is often best – cut perpendicular to long axis at 3 mm intervals
● Always ink margins for tumors, including deep margin
● Bisect punch biopsies if 4 mm or larger

Biopsy for alopecia: can be cut perpendicular or horizontal depending of the pathology

Tzanck preparation (smear):
    - Used to identify Tzanck (multinucleated) cells of herpes simplex, Varicella and herpes zoster, pemphigus vulgaris or cytomegalovirus
    - Scrape the base of the blister / ulcer with the round base of the scalpel
    - Smear on a glass slide
    - Air-dry and stain with Wright's or Giemsa stains (Family Practice Notebook)

Scabies (scrapping):
   - Place a drop of immersion oil on a microscope slide
   - Place a drop of immersion oil on the scalpel
   - Scrape the stratum corneum off the surface of the lesion
   - Numerous lesions should be scraped and placed on the same microscope slide

Submit fresh tissue for:
   - Immunofluorescence (can also use Michel’s medium)
   - Bacterial, fungal, and viral cultures
   - Chromosomal analysis
   - Antigen mapping of basement membrane zone / BMZ (saline splitting)

End of Skin-nontumor / Clinical Dermatology > General > Grossing

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