Cite this page: Fluorescent in situ hybridization (FISH). PathologyOutlines.com website. http://pathologyoutlines.com/topic/lymphomamolecularFISH.html. Accessed August 21st, 2017.
Definition / general
- Used to identify and count chromosomes / parts of chromosomes
- Detects and localizes the presence or absence of specific DNA sequences on chromosomes
- Interphase / metaphase preparation from formalin fixed paraffin embedded tissue can be used
- Fluorescent probes bind to the area of interest on a chromosome; fluorescent microscopes are used to locate the probe
- Various lymphomas carry specific translocations; FISH can detect them rapidly and with high specificity, compared to conventional cytogenetics