Kidney non tumor
Kidney transplantation
Donor evaluation

Author: Nicole K. Andeen, M.D. (see Authors page)
Editor: Maria Tretiakova, M.D.

Revised: 16 November 2016, last major update November 2016

Copyright: (c) 2003-2016, PathologyOutlines.com, Inc.

PubMed Search: Kidney donor evaluation
Cite this page: Donor evaluation . PathologyOutlines.com website. http://pathologyoutlines.com/topic/kidneydonorevaluation.html. Accessed June 23rd, 2017.
Definition / general
  • Performed to determine suitability of allograft prior to transplantation
  • Findings to report: specimen type (wedge or core biopsy), # glomeruli, # and % of globally sclerosed glomeruli, # arteries, interstitial fibrosis, tubular atrophy, interstitial inflammation, arterial intimal fibrosis, arteriolar hyalinosis, other findings (such as FSGS or suspicious lesions) (Am J Transplant 2016 Jun 22 [Epub ahead of print])
  • Biopsy should be of renal cortex, 10 mm long × 5 mm wide × 5 mm deep, and contain at least 25 glomeruli
  • Donor glomerulosclerosis, chronic vascular and interstitial damage are prognostic for graft outcome (Kidney Int 2005;67:1595)
Essential features
  • Demand for donor kidneys far exceeds supply
  • Frozen section may be performed to determine suitability of allograft prior to transplantation
  • Findings to report: specimen type (wedge or core biopsy), # glomeruli, # and % of globally sclerosed glomeruli, tubular atrophy, interstitial fibrosis, interstitial inflammation, # arteries, arterial intimal fibrosis, arteriolar hyalinosis, other findings (such as FSGS, periglomerular fibrosis, etc) (Am J Transplant 2016 Jun 22 [Epub ahead of print])
Epidemiology
  • Demand for donor kidneys far exceeds supply
  • Discard rate of recovered kidneys is > 40%
Prognostic factors
  • Organ Procurement and Transplantation Network (OPTN) recommends preimplantation biopsy for all kidneys with a Kidney Donor Profile Index (KDPI) > 85%
  • KDPI is derived from 10 donor factors: age, height, weight, ethnicity, serum creatinine, history of diabetes, hypertension, hepatitis C virus, cause of death, and donor after circulatory death status
  • KDPI ability to predict graft failure is 0.6; further assessment and pathologic findings may help improve on this (Am J Transplant 2016 Jun 22 [Epub ahead of print])
  • Preimplantation biopsy findings correlate with flow and resistance to machine perfusion (Transplant Proc 2012;44:2197)
  • Expanded Criteria Donor: These are at increased risk of graft failure (relative HR 1.7 compared with Standard Criteria Donor; Clin J Am Soc Nephrol 2009;4:1827)
    • At time of death, donors age > 60 OR
    • Aged 50 - 59 with any 2 of the following:
      • Cause of death is cerebral vascular accident
      • Pre existing hypertension
      • Terminal serum creatinine > 1.5 mg / dL
Case reports
Treatment
  • A decision to implant or discard may be based on the frozen section analysis
Gross description
  • Wedge or needle biopsy
Microscopic (histologic) description
    Recommended microscopic findings to report: (adapted from Am J Transplant 2016 Jun 22 [Epub ahead of print])

    Type of specimen (wedge or core biopsy)
    GLOMERULAR FINDINGS
    Number of glomeruli
    Number of globally sclerosed glomeruli
    Percentage of global glomerulosclerosis
    Other glomerular findings
    TUBULOINTERSTITIAL FINDINGS
    Tubular atrophy, interstitial fibrosis, non-specific interstitial inflammation
    VASCULAR FINDINGS
    Number of arteries
    Arterial intimal fibrosis
    Arteriolar hyalinosis
    OTHER FINDINGS

    • Periglomerular sclerosis and FSGS should be recorded under other glomerular findings
    • Artery: vessel with internal elastic lamina OR diameter greater than one third the diameter of a typical glomerulus cut in the median plane OR a vessel with 3 or more layers of smooth muscle
    • # glomeruli, # and % of global glomerulosclerosis have good reproducibility
    • In most cases, tubular atrophy, interstitial fibrosis, and nonspecific interstitial inflammation are seen together
      • Interstitial inflammation and tubular atrophy are often easier to see than fibrosis on frozen sections
    • Reproducibility is fair for interstitial fibrosis, tubular atrophy, interstitial inflammation, and arteriosclerosis
    • Assessment of interstitial fibrosis and arteriolar hyalinosis is compromised by freeze artifact
Microscopic (histologic) images

Images hosted on PathOut server:

Contributed by Nicole K. Andeen, M.D.

Case 1:

Normal glomerulus (FS)

Normal glomerulus
and adjacent intact
tubular parenchyma (FS)

Focal global GS in
subcapsular region
(H&E perm from FS)

Patches of tubular atrophy
& associated nonspecific
interstitial inflammation
(H&E perm from FS)

Low power with
no global GS (FS)

Low power with focal global GS
predominantly in subcapsular
region and patchy tubular atrophy
and IF (H&E perm from FS)



Case 2:

Focal global GS, ischemic glomeruli
with periglomerular fibrosis, adjacent
tubular atrophy, IF and nonspecific
interstitial inflammatory infiltrate (FS)

Focal global GS, adjacent
tubular atrophy and IF (FS)

Focal global and focal
and segmental GS, tubular
atrophy and IF (perm,
Jones silver stain)

Focal segmental GS, adjacent
arteriolar hyalinosis, tubular atrophy
and IF (perm, Jones silver stain)



Note: FS = frozen section, GS = glomerulosclerosis, IF = interstitial fibrosis, perm = permanent section
Immunohistochemistry
  • On permanent sections, a basement membrane stain (Jones silver, PAS), and thin sections (2 microns) aid in evaluation of potential underlying donor disease (FSGS, diabetes)