Home Jobs Conferences Fellowships Books Buyers Guide
Tissue Processors / Other Histology
for the most recent Promotions page
Tissue Histology: Preserving Information from Molecules
Jaleh Mansouri, M.D.
Tissue processing comprises a basic step in diagnostic pathology but often
involves organic chemicals such as formaldehyde that may destroy the integrity of
molecules. Fixatives like formalin cross-link nucleic acids and proteins. This process
alters the physicochemical structures of these molecules thus rendering them less
amenable to intact retrieval from paraffin-embedded tissue. Macromolecules such as
phosphoproteins and RNA (ribonucleic acid) serve as critical sources of diagnostic and
prognostic information, especially in tumor pathology assays (eg Mammoprint, Oncotype
Dx). While snap freezing is often performed to preserve tissues for molecular analysis,
resulting histomorphology is not optimal and may contain freeze artifacts. For these
reasons, researchers have focused on developing novel fixatives that protect molecules in
paraffin-embedded tissue for future molecular testing.
One example of an innovative technology is the PAXgene tissue system
(PreAnalytiX). This system use formalin-free solution and rapid fixation that aim to
preserve both histology and macromolecules such as DNA and RNA. While this
technology is promising as a tissue processing tool for both histologic diagnosis and
molecular analysis, more studies are needed to evaluate the accuracy and validity of this
technology compared to standard formalin fixation in regard to tissue diagnosis and
Contact Our Advertisers!
Milestone Medical provides a portfolio of standardization and documentation products for the grossing workstation. From our MacroPATH D digital imaging, annotation and storage system to the CheckLITE non-contact specimen thickness measurement device and CutMATE grossing forceps, Milestone is building tools to ensure standardization of tissue thicknesses with great processing and results in mind! For more information call 866-995-5300, email firstname.lastname@example.org or visit us at http://www.milestonemed.com.
Past / Future Feature Pages:
November 2014: Consumable Lab Products / Clinical Lab Analyzers
December 2014: Grossing Equipment / Workstations
January 2015: Billing / Consulting / Management
February 2015: Microscopes / Microscopy Products
March 2015: Tissue Processors / Other Histology
April 2015: Laboratory Accreditation / Education / CME
May 2015: Computer Software and Systems
June 2015: Diagnostic Testing / Reagents
July 2015: Digital / Imaging / Photography
August 2015: Books & Journals
September 2015: Antibodies / Biomarkers
October 2015: Laboratories
November 2015: Consumable Lab Products / Clinical Lab Analyzers
December 2015: Grossing Equipment / Workstations
Contact Sara Halladay-Klakulak, 248/646-0325, email@example.com to advertise at PathologyOutlines.com, the world's most popular pathology website.
End of Feature Page